Journal: International journal of food microbiology

Article Title: Emerging of Shiga toxin-producing Escherichia coli O177:H11 and O177:H25 from cattle at slaughter in Italy.

doi: 10.1016/j.ijfoodmicro.2024.110846

Figure Lengend Snippet: Fig. 1. Heatmap showing the presence/absence of virulence genes (y-axis) within the STEC isolates identified in this study (x-axis). Presence of virulence genes shown in blue, while absence in light green.

Article Snippet: Following the ISO/TS 13136:2012 method (ISO, 2012) each sponge was put into a sterile bag containing 90 ml of modified Tryptone Soya Broth (mTSB; Oxoid) added with novobiocin (Oxoid), homogenised in a stomacher blender for 2 min and incubated at 37 ◦C ± 1 ◦C for 18 to 24 h. After enrichment, a Real-Time PCR was conducted as described in the ISO method using the iQ-Check STEC VirX kit (Biorad, Hercules, CA, USA).

Techniques:

Journal: International journal of food microbiology

Article Title: Emerging of Shiga toxin-producing Escherichia coli O177:H11 and O177:H25 from cattle at slaughter in Italy.

doi: 10.1016/j.ijfoodmicro.2024.110846

Figure Lengend Snippet: Fig. 2. a) SNPs distance matrix results of the STEC O177:H25 strains using A2 as reference. b) Phylogenetic tree based on the maximum likelihood method of the STEC O177:H25 strains. The A2 isolate was used as outgroup to root the tree.

Article Snippet: Following the ISO/TS 13136:2012 method (ISO, 2012) each sponge was put into a sterile bag containing 90 ml of modified Tryptone Soya Broth (mTSB; Oxoid) added with novobiocin (Oxoid), homogenised in a stomacher blender for 2 min and incubated at 37 ◦C ± 1 ◦C for 18 to 24 h. After enrichment, a Real-Time PCR was conducted as described in the ISO method using the iQ-Check STEC VirX kit (Biorad, Hercules, CA, USA).

Techniques:

Journal: Cancer biology & therapy

Article Title: A novel peroxisome proliferator-activated receptor delta antagonist, SR13904, has anti-proliferative activity in human cancer cells.

doi: 10.4161/cbt.8.13.8691

Figure Lengend Snippet: Figure 4. SR13904 exerts inhibitory effects on the cell cycle. (A) A representative experiment in which A549 cells were grown in phenol-red-free DMEM + 2% charcoal-treated FBS ± SR13904 (20 μM). Cells were treated in the exponential phase and assessed by flow cytometry at 24 and 48 h. (B) Statistical analysis of cell cycle distribution (G1, S and G2 phases) from three experiments. All differences (SR13904-treated vs. vehicle control-treated) were highly significant. p values for the G1 phase are shown. **p = 0.001–0.01, ***p = <0.001 (C) Western analysis of select cell cycle proteins. A549 cells were grown in phenol-red-free DMEM + 0.5% charcoal-treated FBS and exposed to the ligand. Relative protein levels were assessed at 24 h and 48 h following addition of SR13904 (20 μM) or control. (D) mRNA analysis of CDK2, CDK4 and cyclin D1. A549 cells were grown and treated as in (C). Real-time PCR for CDK2, CDK4 and cyclin D1 were performed as described in Materials and Methods. Each experimental measurement was normalized to the corresponding GAPDH mRNA levels. For CDK2 (24 and 48 h time points) and cyclin D1 (48 h) time point, the differences (SR13904- treated vs. vehicle control-treated) were significant. *p = 0.01–0.05, **p = 0.001–0.01.

Article Snippet: CDK4 (#2906), cyclin D1 (#2922), cyclin E (#4129), p21 (#2947) and p27 (#2552) antibodies were purchased from Cell Signaling Technology.

Techniques: Flow Cytometry, Control, Western Blot, Real-time Polymerase Chain Reaction